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Teikyo Medical Journal


Journal ID : TMJ-11-06-2022-11309
Total View : 404

Title : Detection of ompF gene in Escherichia coli and it's correlation with antibiotics resistance traits phenotypically.

Abstract :

Porins are part of Outer Membrane Protein (OMP) structure that take parts in passive transport activities of various molecular compounds, one of them is antibiotics molecules. This function caused these proteins often associated with antibiotic resistance in Gram-negative bacteria. The OmpF porin protein is encoded by the ompF gene. The incidence of pathogenic resistance bacteria that causes infection is one of the main problems in the world of health. Escherichia coli is often studied in microbiology field as a model species, especially for the representation of gram-negative group. From the explanation above, it is known that the study of proteins related to antibiotic resistance properties in E. coli bacteria, especially the OmpF porin protein, is very important to conduct. The absence of reports on the protein profile of the OmpF porin in the E. coli group in Indonesia has led to the need to investigate the relationship between the porin protein and the resistance properties of the E. coli phenotype in Indonesia. This study covers the detection of ompF gene in pathogenic E. coli in Jakarta, Indonesia. The detection conducted using amplification by specific primers with conventional PCR method. Results shown that all pathogenic E. coli samples inoculated have the ompF gene. The result supported by the electrophoresis image that shows the DNA bands lied within the estimated amplicon base pair length of around 1200 bp.

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Journal ID : TMJ-10-06-2022-11308
Total View : 380

Title : Teleneurology, Telestroke, and Telerehabilitation in Neurology Health Services

Abstract :

Neurological diseases, such as stroke are the main cause of disability in the world, including in Indonesia. The 2018 Basic Health Research (RISKESDAS) showed that there has been a continuous increase in its prevalence annually. In the era of technology 4.0, hospitals must have telemedicine as a strategic marketing plan to improve their health care services and reach distant patients, including neurology services. This was evident after the incidence of the coronavirus disease 2019 (COVID-19), where they were required to adopt these systems. Several studies reported that telemedicide in neurology services (teleneurology, telestroke, telerehabilitation) can improve the outcomes of patients. Furthermore, distance, neurological deficit disorders, as well as COVID-19 provided the opportunity for teleneurology to become a superior service at the brain and neurosurgery hospitals because it prevents delays in the provision of health care services and aids the continuity at home.

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Journal ID : TMJ-07-06-2022-11306
Total View : 432

Title : The Impact of Vitamin D Deficiency on Some Immunomodulatory Markers in Pregnant Women with Threatened Abortion

Abstract :

The protective mechanism of vitamin D (VD) on the immune system played an important role behind many health problems including abortion. Our aim was to investigate the levels of VD and some immunomodulatory markers (IL-6, TNF-α and INF-γ) in pregnant women with threatened abortion (TA) and their effects on their obstetric health. This study including 132 pregnant participants with TA visiting maternity hospital in Babel province, Iraq. The demographic data including previous and current obstetrical history and body mass index were collected by well-trained researcher following structured questionnaire after obtaining their verbal consent. Laboratory Investigations were done with estimation of VD, IL-6, TNF-α and INF-γ. The mean level of VD was 19.83±43.59 ng/ml while the mean levels of IL-6: 389.45±70.88, TNF-α: 118.78±44.11 and INF-γ: 100.39±20.13 pg/ml with related percentage among participants for low VD was 84%, high IL-6 was 66%, high TNF-α was in 43% but only 23% had high INF-γ in relation with normal values. The frequency of abortion was significantly increased with low VD and high IL-6 (p<0.05) with high correlation between them (r2=0.89). High incidence of TA associated with low level of VD accompanied with high serum levels of IL-6. This adverse pregnancy outcome may be associated with some form of autoimmune diseases with evidence that hypovitaminosis D was correlated with high proinflammatory markers (IL-6, TNF- α and IFN- γ) and worse form of TA and the role of VD is to correct these pro-inflammatory markers.

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Journal ID : TMJ-03-06-2022-11304
Total View : 398

Title : The impact of TCF7L2 gene (rs7903146) polymorphisms on therapeutic response to metformin in Type Two Diabetes Mellitus patients in Al-Dewaniya governorate

Abstract :

Metformin is often used as monotherapy or in combination when diet and exercise are not effective at lowering hyperglycemia. According to the American Diabetes Association, metformin is the preferred drug in newly diagnosed patients with type two diabetes mellitus in adults and children ten years and older. It is recommended as the first-line therapy for type 2 diabetes mellitus (T2DM) based on its efficacy, safety and low cost. In our research, we explored the impact of TCF7L2 gene single nucleotides polymorphisms (rs7903146) on metformin response in T2DM patients in Al-Diwaniyah governorate, Iraq. The current prospective cohort study included 61newly diagnosed patients with type 2 diabetes mellitus. The study was carried out at private clinic, Aldiwaniya governorate/ Iraq. The diagnosis of enrolled patients with T2DM done by an endocrinologist and they were on regular visits to the clinic for follow up. Levels of fasting serum glucose, HbA1c, insulin, HOMA IR, lipid profile, and anthropometric parameters were investigated before start metformin therapy, and 12 weeks after being treated. Genotyping of the TCF7L2 gene SNP was done by RFLP method. There was no significant change in mean HbA1c (p = 0.299); therefore, we chose the cutoff value of < 7% for HbA1c to split the group of patient into two groups, patients with HbA1c of < 7% were considered as glycemic controlled group (n = 22), and the group with HbA1c of ≥ 7 % were considered uncontrolled group (n = 39). We found significant association between TCF7L2 SNP (rs7903146) polymorphism and glycemic control according to an HbA1c cutoff of < 7 % (p = 0.032), in such a way that the heterozygous C/T genotype was more frequent in controlled group than in uncontrolled group, 45.5 % versus 15.4 %, respectively, while, both CC and TT homozygous genotypes were less frequent in controlled group than in uncontrolled group50.0 % versus 71.8 % and 4.5 % versus 12.8 %, respectively. The response to metformin therapy in T2DM patients in terms of glycemic control measured by HbA1c is affected significantly by TCF7L2 SNP (rs7903146 C>T) polymorphism in such a way that the heterozygous C/T genotype define better response.

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Journal ID : TMJ-03-06-2022-11303
Total View : 473

Title : Efficacy of Smart Burs and CARISOLV in caries removal in comparison to conventional method: A Randomized Controlled Clinical Trial

Abstract :

This study was aimed to compare the effectiveness of three techniques of caries removal in primary molars; Smart Burs, Carisolv gel and Conventional Rotary Drill. A total of 60 carious primary mandibular molars were selected. They were equally assigned into three groups; Group 1: Carisolv gel, Group 2: Conventional Rotary Drill, and Group 3: Smart Burs. The time needed to excavate all caries and the pain level caused by each technique were evaluated. Mann-Whitney U test showed that the conventional method needed the least time to excavate all caries, followed by Smart burs and CariSolv, respectively with a statistically significant difference (P<0.05). However, the Chi-Square test for pain values showed a significant difference between the groups in preference to Smart burs group (P<0.05). It was concluded that the conventional method in removing caries needs the least time to excavate all caries, followed by smart burs and CariSolv respectively. In addition, it was concluded that smart burs cause the least pain among the three methods.

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