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19 Apr 2024 (Vol 47 , Iss 04 )

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30 Apr 2024 (Vol 47 , Iss 04 )

Journal ID : TMJ-29-09-2022-11407
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Title : The Effect of Silver Coating Thickness on Cytotoxicity Nickel-Chromium Alloys as Fixed Denture Materials

Abstract :

Nickel-chromium (NiCr) alloy is commonly used for fixed partial denture material. However, these alloys can undergo corrosion which can affect biocompatibility. One of the coatings that can be used is silver. Silver has good biocompatibility, antifungal and minimal allergic potential. This study aimed to examine the effect of the silver coating thickness on nickel-chromium alloys as adhesives on cytotoxicity. The sample consisted of 28 NiCr alloys with disc shape (diameter 5mm; width 2mm), divided into four groups (n=6), namely group I control (NiCr alloy without coating), group II NiCr alloy metal with a silver coating thickness of 1µm, group III is a NiCr alloy with a silver coating of 5µm thickness, and group IV is a NiCr alloy with a silver coating of 10µm thickness. Observing surface morphology and thickness of a silver coating by scanning electron microscope (SEM) used four samples. Cell viability was obtained using the MTT assay method and examined with a microplate reader. The results were analyzed using one-way ANOVA, followed by a post hoc LSD test. The results showed the highest percentage of cell viability in the 5µm thickness silver coating group with a value of 78.93%. The one-way ANOVA and post hoc LSD test showed significant differences between groups (p<0.05). This research concludes that 5µm is the optimal thickness of the silver coating on nickel-chromium alloy as a fixed partial denture material to reduce cytotoxicity.

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