This study was sought to evaluate the opportunities and threats of inactivated polio vaccine (IPV) practices, as well as to develop evidence-based recommendations for policymakers and programme planners. In Qasimabad, Hyderabad, a cross-sectional research was conducted. The study included 300 parents and caregivers of infants who received IPV at seven Expanded Program Immunization (EPI) facilities and outreach sessions, as well as 15 vaccinators and four programme administrators. Data was gathered by convenient sampling with the use of an EPI Monitoring Checklist and a pre-designed questionnaire. SPSS version 23.0 was used for the descriptive analysis. This research identifies the obstacles and areas for development in Pakistan's efforts to eradicate polio and areas of improvements which can be considered for success of program. About 61.7% of parents believed the new vaccination would provide better polio protection, and 65% of babies got IPV on the recommended schedule. Threats were 80% percent of vaccine recipients being male, 46.6 % of parents objected to the third jab, and 50% percent of parents being unaware of IPV. Opportunities include timely IPV vaccination and community acceptance of a novel vaccine; nevertheless, key risks include illiterate moms, a small number of female vaccinators, and a lack of IPV knowledge among parents.