Title : The Effect of Stromal Vascular Fraction to Inhibit Bony Bridge Formation in Growth Plate Injury (Evaluation Using Alizarin Level and Scanning Electron Microscope) in Rattus Norvegicus Rats

The growth plate is important in bone growth. Injury to the growth plate may develop premature fusion or asymmetric growth at the ends of the bones caused by the interaction of bone formation and remodeling in growth plate injuries leading to adverse bone tissue growth, a bony bridge. This study applied Alizarin and scanning electron microscope (SEM) method to measure and compare the bone bridge area in the bones between injured mice had received stromal vascular fraction (SVF) and mice that had not received SFV treatment. An in vitro study using laboratory experimental methods with post-test only control group design. Sample of this research is stored biological material in the form of bone tissue from Rattus norvegicus strain Wistar. Samples were divided into two research groups; the control group was consisted of tissue with growth plate injury minus tissue engineering treatment, and the treatment group received tissue engineering treatment in form of SVF administration. The examination of research samples was carried out in the radiology and biomedical laboratories. Average area of the bony bridge was smaller in the growth plates of rats given SVF compared to those not given SVF either by the Alizarin method or by the SEM method. This has shown that SVF administration can reduce the size of the bony bridge in growth plate injuries. SVF administration can reduce the size of the bony bridge in growth plate injuries on evaluation using an Alizarin staining and scanning electron microscope (SEM).