A smooth, colorless, sweet liquid with a bitter taste and a precise weight of 1.13 is the Honey Bee Poison. It is a complex complex of proteins, carbohydrates, amino acids, and volatile oils that cause stinging pain and heating damages bee venom. The toxin starts to be deposited shortly before its departure from the virgin in honey bee workers' worker bees. For the prevention of rheumatic fever, nerve and joint inflammation and irritation, bee venom may be used. The aim of this study The Effect of Honey Bee Venom (Apis Mellifera) On Pathogenic Bacteria. In the Abbasid district of Salah al-Din governorate, and from the apiary of the College of Agriculture, University of Baghdad, an apiary was discovered. The number of workers ranges from 2000 to 3000 workers in each cell. The extraction was carried out in two ways: the venom of the honey bee is extracted by using an insulin syringe, where the needle is implanted. Direct effect (without dilution) of samples on bacterial and fungi isolates using the cross-drilling method and Specify MIC and MFC. Natural products are known to be a renewable alternative with less problems than a wide variety of active compounds may create. BV includes a number of bioactive ingredients that play a critical role as antimicrobials, including melittin, apamin, and PLA2. BV trials may well contribute to the potential production of novel pharmaceuticals.