Staphylococcus aureus is a gram-positive bacteria that cause a wide variety of clinical diseases. The current work was designed to detect the role of silver nanoparticles as antimicrobial agent. Fifty clinical specimens were collected from patients attending Azadi Hospital, Kirkuk Governorate, the specimens included burn and wound swabs at the period from June 2020 to Murch 2021. The in-vivo experiment was done by using 24 adult male albino mice and divided to four groups. Mice were administrated normal saline as control negative. Mice infected with (104 cell/ml) S. aureus as control positive. Mice administrated with (150 mg/kg) AgNPs for two weeks. Mice infected with S. aureus and treated with (150 mg/kg) AgNPs for two weeks. The results of current study showed the diagnosis of S. aureus by performed morphological and biochemical tests on isolates from wounds and burns, and then the diagnosis of S. aureus isolates was confirmed using API 20E kit. MDA, GSH and catalase show significant (P < 0.05) differences in positive group compared with negative group. In treated group, MDA, GSH and catalase show improvement compared with positive group. So, the current work showed that AgNPs have antimicrobial activity. The current study has research generally concerns the treatment of S. aureus using AgNPs.