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02 Dec 2022 (Vol 45 , Iss 10 )

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30 Nov 2022 (Vol 45 , Iss 09 )

Journal ID : TMJ-27-01-2022-11048
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Title : Serological and Molecular Detection of Rubella Virus In clinical sample of pregnant women and its impact in miscarriage

Abstract :

Rubella virus infection is one of the common viral conditions during pregnancy that may lead to miscarriage, 100 blood samples were collected from a woman with miscarriage and recurrent miscarriage. The patient's age ranged between 15-44 years. ELISA technology was used for serological test which is the primary analysis for rubella. qRT-PCR technique used to determine the type of infection-causing strain due to its high accuracy and short time period in determining the type of strain. The results showed that strains (1E) were more prevalent in rubella patients than strains (2B), which were less prevalent, while strains (1D) didn’t recorded. The results obtained from the serological confirmed miscarriages, of which 48 were distributed over the age groups 15-44 years. As for the search by gene expression, the total number of cases was 87, and the number of miscarriages was 48, distributed between two strains: the first (1E) was 69, and the second (2B) was 18. The third (1D). The strain did not. The difference between the two methods was very slight, amounting to 0.22, where the results were (11.48) in the PCR technique, while it was in the ELISA machine (11.26). For the second table, we found that the number of infections varies with age. And that the age group most exposed to infection was between (24-29) years in the polymerase chain reaction assay, and the first strain (1E) represented (20) positive cases, and the second strain (2B) represented (9) cases. Of the total number (29) of the total number of injuries (93) for all age groups.

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