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27 Jun 2024 (Vol 47 , Iss 06 )

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30 Jun 2024 (Vol 47 , Iss 06 )

Journal ID : TMJ-10-02-2022-11109
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Title : Role of Platelet-Rich Plasma in Repair of the Structural Impairment of Rabbit-Testis Induced by Methotrexate

Abstract :

The aim of this study was to evaluate the effect of platelet rich plasma on the testis structure in infertile rabbit model (infertility induced by methotrexate). This study was performed in Al-Nahrain University / College of Medicine from November 2020 to June 2021. A total of 30 adult male rabbit divided into three equal groups. Ten animals injected with single dose of methotrexate and after 7-10 days, a dose of platelet rich plasma was administered for the testes, ten animals injected by single dose of methotrexate, and ten animals injected with normal saline. Dissection of the Rabbit was done to obtain the testis. The volume of testis was measured. The specimens are kept in 10% formalin for (24-48) hours for histological preparation. There were statistically significant differences in means of lumen diameter, interstitial space, germinal layer thickness, ratio of germinal layer thickness / lumen diameter, leydig cell Number, and sertoli cell number between the three groups. Means of germinal layer, ratio of germinal layer thickness / lumen diameter, leydig cell Number, and sertoli cell Number were significantly higher in PRP group compared to that in other groups, while the means of lumen diameter and interstitial space were significantly higher in MTX group compared to that in other groups. Platelet rich plasma has a potential effect on tissue repair through proliferation and differentiation of tissue progenitor cell and it's simple, safe, easy to prepare and use, easily applicable and effective in using.

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