Klebsiella pneumoniae is a lethal multidrug resistant (MDR) pathogen that affects humans. The bacteria accounts for many hospital infections resulting in great disease and impermanence with imperfect management choices. In hospitalized patients, K.pneumoniae is the primary reason for pneumonia, sepsis and nosocomial infection.The current study employed Viteck-2 sysytem Antimicrobial Susceptibility tests system to assess the antibiotic profile of K. pneumoniae secluded from various medical specimens. Polymerase chain reactions (PCR) of DNA gyrases (gyrA, gyrB) and also DNA topoisomerase parC gene were also conducted to demonstrate the resistant nature of the isolates. K.pneumoniae showed higher resistance towards the penicillin drugs Piperacillin, Ticarcillin and Piperacillin/Tazobactam. Resistance of K. pneumoniae towards cephalosporin antibiotics was tested. Higher resistance (100%) was offered by Cefazolin and Ceftazidime while Cefepime exhibited 80% resistance. Furthermore, the K. pneumoniae isolates exhibited resistance (60.65%) towards carbapenem drugs such as Imipenem and Meropenem followed by aminoglycosides such as Amikacin (45%) and Gentamicin (40%). Resistance towards fluoroquinolones such as Ciprofloxacin (45%), Levofloxacin (55%) and Pefloxacin (65%) were observed. The PCR detected that the fluoroquinolone resistance genes gyrA (25%), gyrB (30%) and parC (20%) were all present the isolated K. pneumoniae strains. This study confirmed the drug resistance of K. pneumoniae strains and demonstrated possible factors responsible for their resistive nature.