Submission Deadline
20 Jun 2024 (Vol 47 , Iss 06 )

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30 Jun 2024 (Vol 47 , Iss 06 )

Journal ID : TMJ-15-02-2022-11128
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Title : Molecular study of theophylline effect on the neural stem cells differentiation in vitro

Abstract :

The current study was conducted to the isolation and culture of the neural stem cells (NSCs) get from the brain of mice (Mus musculus). NSCs were isolated from the brain of mice embryo at the age of 17 days, and investigated their growth and differentiation. These cells were cultured in specific media, DMEM/Ham’s F12, in addition to the growth factors (recombinant human epidermal growth factor (rHuEGF) and fibroblast growth factor-basic (FGF-2)). NSCs were incubated at a temperature of 37°C with 5% CO2. After 24 hours the spherical-shaped NSCs were demonstrated with high nucleus to the cytoplasm ratio. Also these cells exhibit a high activity of multiplication and this may indicates the key role of the growth factors in the elucidating of cellular proliferation and multiplication. In addition, cells tend to aggregated to form a small groups of primary neurosphere, which consists of two to three and four cells, and this reveals the initial step in the formation of the neurosphere. On the other hand, the ability of theophylline on the NSCs differentiation were inspected, and the cytotoxicity of theophylline effect on the survival and activity of the NSCs were studied by MTT test. The marks of this test indicated that the theophylline has no cytotoxicity at the concentrations used in this project. Also, the results showed that theophylline had no significant effects on the differentiation of the neural stem cell. While, the consequences of the RT-PCR were consistent with the morphological change, that cells maintained their high gene expression matching no differentiation had taken place. It could be concluded that the theophylline has no cytotoxic effect on NSCs at the doses employed in this project, and also it has no impact on the differentiation of NSCs.

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