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25 Apr 2024 (Vol 47 , Iss 04 )

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30 Apr 2024 (Vol 47 , Iss 04 )

Journal ID : TMJ-23-09-2021-10662
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Title : Molecular evaluation of BK and MC human polyomavirus as promising Prostate cancer markers in Moroccan population.

Abstract :

Prostate cancer (PCa) is one of the leading causes of cancer deaths in men worldwide. Current evidence of an oncogenic role has emerged for Merkel cell polyomavirus (MCPyV) and BK human polyomavirus (BKPyV). The aim of our study was to describe the molecular evaluation of BKPyV and MCPyV as promising PCa markers and to investigate their correlation with clinical features of the tumor in Moroccan population. Fresh prostate biopsy tissue specimens were from 50 patients with confirmed PCa. The DNA extraction was performed by Invitrogen Genomic DNA Mini Extraction Kit The quality of DNA was checked using Nano drops test. BKPyV and MCPyV genomes were molecularly characterized using polymerase chain reaction (PCR) methods. The PCR products were subjected to the gel electrophoresis. BKPyV and MCPyV were detected with a prevalence of 24% (12/50) and 0% (0/50) prostate tissue specimens, respectively. PCa Gleason scores 7 was the most cancer grades identified with the presence of BKPyV (p<0.05). We suggest that BKPyV and MCPyV confers a risk for PCa, and patients with Gleason scores 7 are at a greatly increased risk in Moroccan population.

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