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02 Dec 2022 (Vol 45 , Iss 10 )

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30 Nov 2022 (Vol 45 , Iss 09 )

Journal ID : TMJ-29-01-2022-11059
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Title : Molecular diagnosis and genotyping of cystic echinococcosis from clinical samples of human in central and northern Iraq

Abstract :

The study included 45 samples of hydatid cysts isolated from humans for the period from April 2021 to October 2021 from hospitals in central and northern Iraq. This study was carried out at Almusaib bridge for scientific and lab equipments, The study aimed at the molecular diagnosis of Echinococcus granulosus, the identification of the causative strains of hydatid cyst disease using the polymerase chain reaction technique, the study of genetic sequencing of the strains, and the drawing of the phylogenetic tree to know the convergence of the strains causing the infection in the study area with the strains spread in the rest of the world , DNA was extracted from the protoscolices and the germ layer and amplified by the polymerase chain reaction technique. The COX1 gene with a molecular weight of 444bp was amplified. By analyzing the sequence of nucleotides for 17 samples of the cox1 gene and analyzing the phylogenetic tree and comparing it with the gene bank, it was found that the sheep strain (G1) is the strain that causes infection in humans in central and northern Iraq, The observed variations were respectively deposited in the NCBI-bank it database under the accession numbers (OL614464 to OL614480) to represent S1, to S17 samples respectively.

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