Entamoeba histolytica is an anaerobic parasitic amoebozoan, part of the genus Entamoeba. Predominantly infecting humans and other primates causing amoebiasis, E. histolytica has an estimated worldwide prevalence of 500 million infected and is responsible for 40,000-100,000 deaths each years it is an important health problem, especially in developing countries. The present study was conducted to molecular detection of E. histolytica by specific primer. A total of 100 stool samples from the patients (60 males, 40 females) age range between 8-55 years from March to August 2020. The collected fresh stool samples were microscopically examined using the direct saline/ iodine wet mount microscopy to detect Entamoeba trophozoites and/or cysts. About 0.2 g of each specimen was preserved at –20°C for molecular analysis. The present study was showed the proportion of male patients with diarrheal illness was more than that of female subjects, 60 versus 40, respectively. In the current study, only 24 (34.3 %) were proved to have E. histolytica infection out of the 70 cases who were diagnosed based on the morphology. The imminent expansion of the amount genome data will greatly improve our knowledge of the genus and of pathogenic species within it.