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Journal ID : TMJ-05-08-2021-10539
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Title : Mechanism of action of Polycephalomyces nipponicus and Ophiocordyceps sobolifera mycelial extracts against Methicillin-Resistant Staphylococcus aureus using electron microscopy

Abstract :

The mycelial extracts of Polycephalomyces nipponicus and Ophiocordyceps sobolifera have been shown to possess antibacterial activity in several recent studies. This study aimed to examine the mechanism of action of both medicinal fungal mycelial extracts against Methicillin-resistant Staphylococcus aureus (MRSA) DMST 20651 and Methicillin-resistant Staphylococcus aureus (MRSA) DMST 4738 compared with linezolid and vancomycin antibiotics by using scanning (SEM) and transmission electron microscopy (TEM). SEM analysis showed that both isolates of MRSA treated with 1× minimal inhibitory concentration (MIC) of both mycelial extracts had varied in cell size. While the TEM analysis showed that the cell shape is not round and distort, bacterial cell walls and cell membrane were altered and damaged, leading to cell death. Similar results were observed in the treatment of linezolid and vancomycin. These results suggested that P. nipponicus and O. sobolifera mycelial extracts exhibited to inhibit bacterial growth by disrupting peptidoglycan formation, which is a component of the cell wall and resulted in cell death.

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