The results of numerous studies have revealed that cancer cell growth has been affected in response to treatment with anticancer agents, also some cancer related antigen were affected (decrease or increase) as a results when the cancer cell were treated with anticancer agents, in the present study, the in vitro cytotoxicity effects of different drug (5-fluorouracil, curcumin and feboxustat) was measured by using crystal violet technique, the results showed significant (p≤0.001) anticancer activity on LS174T colorectal cancer cell line at different concentrations. Also for the same drugs (5-fluorouracil, curcumin and feboxustat) Carcinoembryonic antigen (CEA) level was measured by using ELISA technique, the results showed significant (p ≤0.001) decrease in CEA activity at different concentrations. Study the cytotoxic effect of effect of 5-FU, Curcumin and Febuxostat on colorectal cancer LS174TCell Line, Study the effect of 5-FU, Curcumin and Febuxostat on the level of Carcinoembryonic antigen (CEA expression). 5-FU, Curcumin and febuxostat when used, showed good anti-cancer activity on colorectal cancer LS174T cell line. On CEA expression, the 5-FU, Curcumin and febuxostat when used, cause a decrease in the expression of CEA levels suggesting tumor shrinkage and anti-cancer effect.