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07 Jul 2024 (Vol 47 , Iss 07 )

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Journal ID : TMJ-03-01-2022-10948
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Title : Isolation and Identification of Endophytic Fungi Producing of Antioxidant Compound from Azadirachta indica A.juss Based on gen 18s rRNA

Abstract :

Endophytic fungi are microbes living in plant tissues and are currently being explored as an alternative to bioactive compounds because they produce metabolites usable as medicinal raw materials. Therefore, this study aims to isolate and identify endophytic fungi that produce antioxidant compounds from neem (Azadirachta indica A.juss) leaf. The isolation process produced 4 endophytic fungi coded as FDM 1, FDM 2, FDM 3, and FDM 4. Subsequently, the isolates were fermented for 10 days using PDY media, and then extracted using ethyl acetate solvent in a ratio of 1:1. The TLC method detected the antioxidant compounds in the extract, and FDM 2 was observed to contain antioxidant compounds, which was indicated by yellow spots on the stains after spraying with DPPH. Furthermore, the antioxidant activity test showed that FDM 2 has an IC50 value of 54.93 ppm, while the molecular identification results based on the 18s rRNA gene showed that FDM 2 was similar to Lentinus squorosulus with an identity percentage of 99.55%.

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