Title : Inhibition biofilm former Candida albicans that causing oral thrush by purified biosurfactant(sufactin) from dental decay Lactobacillus acidophilus

One of the most common dental ailments, dental caries is caused by a long-term infection of the mouth's natural flora. Sugar-eating bacteria in the mouth produce acidic compounds that cause cavities. Six isolates Lactobacillus fermentum, Lactobacillus acidophilus and Lactobacillus plantarum isolated from dental caries reveled different levels in biosurfactant productivity with emulsification indexes (E24%) ranged between 6-18% and emulsification activity(E.A.) values ranged between 3.6-7.2. An adsorption chromatography silica gel column were used to purify of biosurfactant then characterized as lipopeptide by thin layer chromotography. Eight Candida albicans was obtained from individuals afflicted with oral thrush and all these isolates formed biofilm, but at varying amounts. The purified biosurfactant revealed antibiofilm activity against all Candida albicans isolates with percentage in biofilm inhibition ranged between 44-70%. So that Candida albicans-infected oral thrush can be treated with biosurfactant.