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25 Apr 2024 (Vol 47 , Iss 04 )

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30 Apr 2024 (Vol 47 , Iss 04 )

Journal ID : TMJ-31-10-2021-10767
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Title : Impact of Sperm Preparation Techniques on the Sperm DNA-Fragmentation, Concentration and Motility

Abstract :

The effect of sperm quality and optimal sperm preparation techniques on assisted reproductive techniques success are still under debate. To assess the effect of sperm preparation technique on sperm DNA fragmentation, motility and concentration. A prospective study conducted at the infertility consultation clinic in Kamal Al-Samarraee Hospital for infertility and IVF, Baghdad / Iraq, included30 Iraqi infertile males who met the inclusion criteria from them thirty semen samples were collected. Semen samples were fresh, 1.5 ml volume, at least, sperm concentration ≥ 20 x 106 / ml and total sperm motility ≥ 30%. Samples taken after abstaining from ejaculation for 2-5 days. After baseline evaluation and analysis, all the selected semen divided into two sample each of them managed with either pellet or semen swim-up technique. Then we tested for concentration and motility of sperms in addition to DNA fragmentation. Mean DNA fragmentation rate was significantly lower in direct swim-up than pellet swim-up technique, 27.3% ± 7.9% vs. 14% ± 9.6%, (P=0.001). Moreover, it had been significantly. The median sperm concentration was significantly higher in pellet swim-up, 60%, compared to direct-swim-up with 35%, (P. value = 0.001). Other parameters included the progressive, non-progressive motility, morphology and immotility of sperms were not significantly different, (P. value > 0.05). Direct swim-up sperm preparation technique was better than pellet swim-up technique in reduction of sperm DNA fragmentation and sperm concentration where it was higher in pellet than direct swim-up technique.

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