Many patients with Laryngeal squamous cell carcinoma (LSCC) do not have any of the traditional risk factors associated with head and neck squamous cell cancers. Epidemiological and molecular studies have identified human papillomavirus (HPV) as a causative agent. The aim of the study is to find the relation between HPV-16 infected LSCC and T and N stage of the tumor. Prospective, cross section Tertiary university hospital. The current study was conducted on 47 cases suffered from LSCC, all patients subjected to clinical, radiological and endoscopic assessment of the tumor. Biopsy was taken from each patient and stained by H&E to confirm the clinical diagnosis and also Immunohistchemical staining was done to evaluate infected tumors by HPV-16. SPSS program version 16 was used to assess the correlation between HPV-16 affected tumors and T and N stage of the LSCC. The study sample pointed out that the majority of patients were in T2 and N0 stage. The current study showed that most of the patients infected with HPV-16 presented in T2 stage (six out of nine patients 66.7%), while the majority HPV-16 negative patients also presented in the same stage (36 patients 94.7%). There is no difference between HPV-16 positive and negative patients in N stage as the majority of both groups presented in N0, 66.7% in positive cases compared to 76.3% in negative cases. Our results reveal that 22.2% of HPV positive cases were in stage T3 and T4 compared to 0% in the HPV negative cases in the same stages. The second common presenting N stage of HPV-16 positive cases was N2, while in HPV negative patients was stage N1. Our results showed that there were no correlation between T nor N stage of HPV-16 infected LSCC, but indeed not statistically significant we found that HPV positive cases tend to be presented more in advanced T and N stage compared to HPV negative cases.