E-cadherin is a protein that plays a role in cell adhesion and regulates the stability of contact interactions at the cell surface. E-cadherin production may decrease in cases of C. albicans infection, which may affect the integrity and stability of vaginal epithelial cells. A total of 48 rattus norvegius wistar rats were divided into 4 positive control groups (P1) were given topical C. albicans after being grown in yeast extract peptone dextrose (YPD) in the vagina of rattus norvegicus wistar white rats. Groups P2 & P3 were treated with Holothurin 3500 ug and caspofugin 140 ug topically on the vagina of the animal model in 12-, 24- and 48-hours intervals. Immunofluorosens was performed to analyze the results quantitatively using imageJ software and qualitatively by listing the imaging. Data were then processed using SPSS version 23 statistical software. Measurement of E-cadherin expression after VVC for 12, 24, and 48 hours showed no significant difference in all groups. The negative control group had lower E-cadherin expression than the treatment groups, and in the C. albicans positive treatment group, E-cadherin expression increased over time. The holothurin-administered group showed suppression of E-cadherin expression at hours 12 and 24, however at hour 48 E-cadherin expression rose slightly although not significantly. Holothurin given to experimental animals with candidiasis has significant changes in suppressing the amount of E-cadherin in the vaginal epithelial tissue of wistar rattus norvegicus.