Abstract :
Recently gut microbiota became implicated in the pathogenesis of obesity. Gut microbiota not only digest food but also regulate our immune system, produce vitamins and aggravate insulin resistance. Firmicutes and Bacteroidetes are the dominant bacteria, accounting for approximately 99% of the whole microbiota. To clarify the relationship between obesity and gut microbiota (bacteriodetes and firmicutes types). This study was done in Mansoura Specialized Hospital, Mansoura University during the period from May 2019 to May 2020. Two hundreds patients were included and divided according to body mass index (BMI) into three groups (ideal body weight group, overweight group, and obesity group). All included subjects will undergo history and clinical examination, abdominal ultrasound for obese group, measure body mass index (BMI), waist circumference (WC), and waist-to-hip ratio (WHR). Blood samples analyzed for complete blood lipogram, liver functions, and fasting blood glucose level, CRP. Moreover, stool sample for microbiota study (Bacteroidetes and Firmicutes) by polymerase chain reaction (PCR). There was higher microbiota in obese group than ideal and overweight. Firmicutes was associated with NAFLD, higher CRP, ALT and bilirubin. Higher BMI and lower TC were associated with increased likelihood of exhibiting microbiota with sensitivity 87.9%, specificity 45.5%, PPV 72.2%, and NPV 70%. Microbiota mostly play an important role in pathogenesis of obesity and its complications including metabolic syndrome and fatty liver especially Firmicutes type. This can be possible strategies for treatment of obesity.
