Virus SARS-CoV-2 attaches to host cell surface receptors via the spike glycoprotein (Sgp). As the key target for vaccine and treatment studies, it may be important to explain Sgp mutation. These variants carry different mutations that can modify the pathophysiology, transmissibility, and efficacy of the currently available vaccine. The aim of our study is to discover the relationship between certain residues and the Sgp SARS-CoV-2 functioning through the use of explorative interpretation of sequences. Here, we analyzed 40 SARS-COV-2 sequence samples in the Spike protein region which were uploaded into the GISAID public database from March 2021 to the end of 2021. Our data shows that B.1.1.7 (Alpha) variant was a predominant lineage of the virus in Iraq, following by Delta variant (B.1.617.2 lineage, 16%), and sublineage of Delta variant AY 3.3 (11%). The site mutation D614G was found among all the sequenced sample (100 %), followed by P681H (65 %), N501Y (60 %), A570D (60 %), S982A (60 %), D1118H (60 %). The continual development and emergence of multiple variations of the virus in the region, as evidenced by our results, is significant. The findings from this study should assist drug industry in developing an effective vaccine to overcome the COVID virus.