07 Dec 2022
30 Nov 2022
Toxoplasma is a dangerous parasite that exists inside the cells of the human body, which leads to serious cases such as abortion in pregnant women. In this study, a total of (60) blood samples were collected from aborted women who attended Azadi Hospital/ Kitkuk City-Iraq, during the period from 1st February to 1st September 2021. The results showed that the mean age of patients was (41.84±15.88) years, while in the control group it was (41.84±15.88) with no significant difference P>0.05. The mean of anti- Toxoplasma IgM was (2.00±1.17) in the patients group in comparison with the controls (0.09±0.17), while the mean of anti- Toxoplasma IgG was (14.20±7.06) in the patient women compared to the control group (0.06±0.11) with a highly significant difference. According the residency of the study group, the mean of anti- Toxoplasma IgM in urbans was (1.95±1.25), compared to the rural citizens (2.05±1.10), and the anti- Toxoplasma IgG in urbans was (14.86±6.71) in comparison with the rurals (13.55±7.43), with no significant difference. There was a highly significant difference between the anti- Toxoplasma Antibody IgM (2.00±1.17) and the control group (0.09±0.17), and between anti- Toxoplasma Antibody IgG (14.20±7.06) and the control group (0.06±0.11) and between the IL-1β level (56.84±18.87) and the control group (88.09±14.85). There was also a highly significant difference between the mean anti- Toxoplasma Antibody IgM (2.00±1.17) and the mean anti- Toxoplasma Antibody IgG (14.20±7.06). The mutation occurrence in IL-1β gene (rs 1143634C>T, the complete nucleotide sequence of the amplified exon in the region was determined. The cytosine was substituted by thymine at the site (rs 1143634) in toxoplasmosis. Expression of IL-1β gene was investigated in Toxoplasma gondii among aborted women and healthy controls using qRT-PCR, and the findings of amplification were explained showing atypical amplification plot. Amplification reaction has an early threshold cycle that is consistent with highly levels of IL-1β gene and healthy controls.