02 Dec 2022
30 Nov 2022
Human cytomegalovirus (HCMV) is the most frequent cause of congenital infection in humans. Infection with HCMV is very common worldwide with seropositivity rates ranging from 40% in developed countries up to 99% in developing countries. Toll-like receptors (TLRs) play a crucial role as a first line of host immune defense against (HCMV). Owing to the high percentage of CMV among aborted women with recurrent miscarriage, therefore, the gene polymorphisms of Toll- like receptor (TLR-2 and TLR-9) in CMV aborted women was examined by using a high resolution melting PCR. Seventeen cervical swabs out of 54 were positive for CMV obtained from aborted women from Baghdad maternity hospitals and seventeen cervical swabs as a control group obtained from healthy women for ages (17-40) years old. The result of gene polymorphism of TLRs (TLR-2, SNP rs-5743708 and TLR-9, SNP rs-352140) in CMV aborted women, showed that the homozygous mutant A/A appeared in 58.8% (OR= 22.8571, CI= 2.4349 to 214.5638, P=0.006). The is indicate the significant role TLR-2 A/A genotype in CMV escape from immunity of aborted women. Whilst TLR-9 SNP in CMV infected women resulted in 52.9% of patient had a heterozygous mutant C/T (OR= 39.1176, CI=2.02779 to 754.5664, P=0.015). These results indicated that TLR-2 mutant SNP had a significant impact on CMV aborted women than TLR-9 SNP. In conclusion, genetic polymorphisms of TLR-2 had a significant role in CMV infected women, rather than TLR-9 SNP, which many correlated with miscarriages in Iraqi women.