Title : Gene Expression of Leishmania donovani Exosome in BALB/c Mice by Real Time qPCR

The aim of this study is to molecular detection of Leishmania donovani exosome by real time PCR gene expression. The results shows that the mean of gene expression in spleen tissue was (10.3300), in passage 1, while the mean of gene expression of was (24.6733), in passage 3. The control culture line, the mean of gene expression of was (8.0833), in passage 1, and (0.2300), in passages 3. This study explained that exosome gene expression was higher in spleen than in cultures media. The higher levels were in passage 3 and lowest levels in passage 1. The level of gene expression was declined in control culture line were the higher levels were in passage 1 and lowest levels in passage 3.