Proteus mirabilis bacteria have a unique ability to contaminated wounds and lead to severe host tissue damage. The aimed of our research to evaluate the antibacterial efficacy of nickel ferrite nanoparticles alone and in mixed with antibiotic against multidrug-resistant Proteus mirabilis utilizing Kirby-Bauer and molecular methods. included collection of 50 burned wound sample from patients in Tikrit Teaching Hospital and external clinics. (from October 2018 to March 2019). results revealed that 40(80%) from samples were react positively for Proteus mirabilis. Bacterial isolates (Proteus mirabilis) Doxycycline hydrochloride, Penicillin, CO-Trimoxazole, Ciprofloxacin, Cephalosporin, and Penicillin resistance were found. results also revealed the (NiFe2O4) inhibits strongly growth of bacteria isolated at various mini-mum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) The MBC concentricity equal 256 while the MIC concentration was 128(mg.I-1) (mg.I-1) signifi-cant alterations recorded within DNA were observed before and after processing with nano-particles, according to the findings.