Urinary [IGFBP7×TIMP-2] involved in G1 cell cycle arrest during the early phases of cellular stress or injury caused by various insults (e.g., oxidative stress, sepsis, ischemia and toxins). This Cross-sectional Study was designed under the supervision of College of Medicine, Almustansiriya University, Chemistry and Biochemistry Department. A 176 patients, 88 patients with primary renal stone, and 88 patients with secondary renal stone. Urinary [IGFBP7×TIMP-2] level in primary renal stone group was significantly lower than of secondary renal stone group. p ≤0.0001, AUC for [TIMP-2 × IGFBP-7], was 1.000, primary renal stone group has urinary [TIMP-2 × IGFBP-7] level lower than 1.93620 (ng/ml)2, secondary renal stone group has urinary [TIMP-2 × IGFBP-7] level higher than 1.93620 (ng/ml)2. Urinary [IGFBP7×TIMP-2] in both of primary renal stone group and secondary renal stone group were a perfect markers for predicting Renal stone disease in patients with acute kidney injury.