The current study was carried out in order to gain an understanding of the diverse microorganisms linked with trash dump soil and their antimicrobial resistance profiles in four hospitals in Baghdad. All the isolates' morphological, biochemical, and physiological features were analyzed. A systematic morphological, and biochemical identification was carried out once isolated colonies were isolated from each sample. From the results obtained it was observed that Klebsiella oxytoca. Staphylococcus aureus, E.coli, Providentia were predominant organisms. The standard disc diffusion method was used to perform antibiotic susceptibility testing for isolates. We studied the three major organisms found in the soil namely staphylococcus, e.coli and klebsiella. Fortunately most of them were sensitive to the common drugs used in hospitals except for klebsiella, which showed weak resistance to the drug piperacillin. Ciprofloxacin was the most potent agent on the isolated bacteria, followed by Augmentin and Gentamicin in hospital solid waste, respectively. Most of the isolates were sensitive to common drugs. DNA was extracted from every organism isolated in this study and amplified via PCR followed by purification and Genome sequencing. The sequences were then analyzed to identify the bacterial isolate.