Trichomoniasis is the most prevalent sexually transmitted disease(STD) non-virus caused worldwide. The current study aim to detection of T.vaginalis infection in vaginal secretions and urine samples to studying its relationship with other types of bacterial and fungal pathogens associated with infection with this parasite, as well as finding the relationship between demographic factors and research results. In this study the vaginal wet mount for 232 females were collected and urine samples from same patients as well as 25 samples from healthy female (control) were collected with different ages ranged between (14-58) years who attended the hospitals and medical clinics from different regions of Karbala province from the period Nov.1, 2020 to June.5, 2021. Wet mount preparation, direct Gram smear, and GUE results showed the highest infection rate was 02 (8.62%) with T.vaginalis, general exam was the best test used in this study to detected the T. vaginalis. The statistical analysis was done by using statistical package for social science program (SPSS version 24), Chi-Square test (χ2) was used to determine relationship between the infection rate and variables that used in the current study and percentage, with a probability (p) value of 0.05 or less were considered as statistically significant.