Submission Deadline
18 Aug 2022 (Vol 45 , Iss 06 )

Upcoming Publication
31 Aug 2022 (Vol 45 , Iss 06 )

Journal ID : TMJ-28-12-2021-10931
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Title : Enhancing the antibacterial activity of Thymus kotschyanus essential oil with Pseudomonas fluorescens as bioinoculant against some pathogenic bacteria

Abstract :

Inoculation of medicinal plants by Plant Growth-Promoting Rhizobacteria (PGPR) improves plant characteristics, essential oils (EOs), and secondary antimicrobial metabolites. To evaluate the antimicrobial activities of Thymus kotschyanus (T. kotschyanus) treated with rhizobacteria Pseudomonas fluorescence (P.flourescence). The characteristics, including fresh and dry root mass and height of T. kotschyanus, were assessed with or without P.flourescence strain Pf169 treatment. GC-mass GC/MS were used to analyze the antimicrobial metabolites of T. kotschyanus's EOs with and without Pf169 treatment. Antibacterial effects of Pf169-treated T. kotschyanus were evaluated by agar diffusion method against tree multidrug resistance (MDR) Acinetobacter baumannii, Pseudomonas aeruginosa, and Staphylococcus aureus. The results showed an improvement in T. kotschyanus after Pf169 inoculation. Furthermore, Pf169 inoculation has led to increased levels of monoterpenes. Antimicrobial compounds in T. kotschyanus's EOs were active against three pathogens. P. fluorescence modulated T. kotschyanus's EOs and its secondary metabolites can be used as an option against MDR bacteria alone or combined with common antibacterial agents.

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