Abstract :
Hemorrhagic cystitis is often associated as an effect of cyclophosphamide (CP) usage in the treatment regimens of malignant and non-malignant diseases. Indonesia has various medicinal plants that can be used as therapeutic developments, one of which is Moringa (Moringa oleifera L.). Moringa leaf is part of the plant that is mostly used and contains flavonoids that function as antioxidants. The purpose of this study was to prove the prevention effect of Moringa leaf extract administration on TNF-α and IL-10 levels in hemorrhagic cystitis after CP administration. The research used post-test control only group design. Six-month-old Wistar rats (Rattus norvegicus) with a body weight of 150-200 grams, were divided into 3 groups. Control group 1 (K1) was only given 0.9% NaCl orally during the study period, Control group 2 (K2) was given 0.9% NaCl orally and CP, Treatment group (P) was given Moringa leaf extract at a dose of 500 mg/kgBW orally and CP. Each oral doses were given for 7 days before, 1 hour before, and 4 hours after injection of single dose CP at 200 mg/kgBW intraperitoneal. Rat serum was obtained 24 hours after all treatments and followed by examination of TNF-α and IL-10 levels. Based on the data analysis, there was a significant decrease in TNF-α levels and a significant increase in IL-10 levels in rats group given 500 mg/kgBW Moringa leaf extract. Moringa leaf extract at a dose 500 mg/kgBW gives prevention effect by reducing TNF-α levels and increasing IL-10 levels in hemorrhagic cystitis after CP administration.
