Abstract :
Periferal Arterial Occlusion Disease is obstructive artery disease in lower extremity because of decreasing arterial flow when activity, and in advanced case, it happened also in resting. Because the occlusion, there was dereasing blood flow to the distal with the effect of hypoxia. This situation will stimulate Vascular Endothelial Growth Fator (VEGF). VEGF will stimulate angiogenesis. Endothelial Nitric Oxide (eNOS) also have important impact in angiogenesis process. Platelet also have impact, when the platelet is activated and aggregated by hypoxia, can stimulate VEGF to start angiogenesis cascade. Analyze PRP effect in eNOS expression, angiogenesis, and necrotic area in ischemic condition because vascular lesion. This study is an experimental research. The study samples are orcytolagus cuniculus with weight 700-1000 grams, male, age 8-10 months with acute ischemic in lower extremity because vascular lesion. The samples will divide in 2 groups, one groups have PRP injection and the other one not. Every group will divide again to 4 groups with different ligation time, 2,4,6, and 8 hours. There was significant difference in eNOS expression, with PRP group has better outcome in 2,4,6, and 8 hours ligation and the correlation is strong and directly proportional with ligation duration. There was significant difference in angiogenesis, with PRP group has better outcome in 2,4,6, and 8 hours ligation and the correlation is strong and directly proportional with ligation duration. There was significant difference in necrotic area, with PRP group has better outcome in 2,4, and 6 hours, but didn’t significant in 8 hours ligation and the correlation is strong and inversely proportional with ligation duration. PRP significantly increasing eNOS expression and angiogenesis, and decreasing necrotic area in inchemic lesion because vascular lesion.
