Endometrial cancer is one of the most common cancers worldwide. The incidence and mortality due to this cancer increase continually, especially in middle- and low-income countries. Most endometrial cancers are driven by estrogen-related hormonal factors. However other possible risk factors such as infection might also be involved in this disease. Our objective is to investigate the possible implication of Human PapillomaVirus in Endometrial cancer. In this study, we used nested PCR with HPV L1 consensus primers (MY09/11, and GP5+/6+) to investigate the presence of HPV L1 sequences in 71 endometrial cancer tissues and 53 normal controls. To determine HPV genotypes, type-specific PCR for HPV 6, 11, 16, 18, 31, and 33 was carried. HPV L1 sequences were detected in 11 cancer specimens (15.49%) and 4 normal samples (7.54%). The genotypes detected were HPV16, HAPV30, HPV6 and HPV11. Our results showed no significant association of HPV infection to endometrial cancer.