Kediri District is one of the Districts in East Java Province, Indonesia, that is categorized as an area with Extraordinary Occurrence for Dengue Fever. In 2015, it was occurred double increase for dengue fever cases rather than in 2014. The distribution of domicile for Dengue Hemorrhagic Fever sufferers in Kediri District in 2016 was mostly in Pare Subdistrict and the sufferers were 107 sufferers. Then, the next subdistrict was Ngasem Subdistrict, which was 90 sufferers, and Kunjang Subdistrict, which was 74 sufferers. Vector control of Dengue Hemorrhagic Fever for a stadium of adult mosquito uses active ingredient of Malathion. Resistance of vector against insecticide is a global phenomenon and an obstacle to the success of vector control chemically. This research aimed at analyzing detection conventionally regarding the resistance of Aedes aegypti mosquito as Dengue Hemorrhagic Fever vector in Kediri District against Malathion. This research was true experimental research and the procedures of examination in this research used WHO standard of Susceptibility test through using impregnated paper with Malathion dose in 0,8%, 0,5 %, and 0% (control variable). Data analysis referred to resistance status from WHO standard and it analyzed the difference of experimental biota death by using the statistic of ANOVA difference test. Research results showed that Aedes aegypti mosquito in Kediri District was resistant against malathion 0,8%, meanwhile, the use of malathion 5% was in the category of tolerance of 60 minutes. There was a significant influence between contact time and death of the Aedes aegypti mosquito. Suggestion for Health Office in Kediri District was the use of malathion as insecticide needed concentration in more than 0,5%.