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20 May 2024 (Vol 47 , Iss 05 )

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31 May 2024 (Vol 47 , Iss 05 )

Journal ID : TMJ-05-02-2022-11092
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Title : Correlation between IL-1β, IL-18 and some hematological variable in uropathogenic E. coli infected UTI patients

Abstract :

Urinary tract infection (UTI) is one of the most common types of infection acquired from society and hospitals, so the current study aimed to know the extent of this infection and to detect the causative bacteria, as well as to know the effect of urinary tract infections on some blood and immune indicators represented by interleukin 1 beta (IL-1β) and interleukin 18 (IL-18). The relationship of some hematological and immunological indicators with urinary tract infection and the study of the relationship of IL-1β and IL-18 with each other on the one hand and with some hematological indicators on the other hand was studied, which is a first study of its kind. In this study, 77 blood and urine samples were collected from patients with urinary tract infection, while 10 samples were taken from healthy patients without UTI and who do not suffer from chronic diseases and they were used as a control group. All urine samples were examined and cultured, in addition to conducting laboratory blood tests to identify blood variables, and the level of some immune variables, which included IL-1β and IL-18, was measured using the Enzyme Linked Immune Sorbent Assay (ELISA) technique. The results obtained from urine culture, 61 out of 77 samples recorded bacterial growth (positive result) and 16 samples did not record bacterial growth (negative result), while all healthy samples were culture negative, 4 types of bacteria causing urinary tract infection were isolated. In our study, S. aureus bacteria constituted the largest percentage, 57.4%, followed by E. coli bacteria, 24.6%. The current study also showed that the most affected age group of adults is the group (15-29 years), with a rate of 37.7%. When evaluating the level of IL-1β in patients and comparing it with the healthy group, a significant increase of 0.0957 pg./ml was found in the patients compared with the healthy controls. And when evaluating the level of IL-18, a significant increase was found at a rate of 0.1107 pg./ml in patients compared to healthy controls with a lower level. With regard to blood variables, an increase in the level of white blood cells was observed (7.82 ±3.32) in the affected patients when compared with the control group (6.71 ±1.01). As for the other variables, non-significant differences were noted when comparing patients with the healthy ones. The interrelationships between the variables were studied. Hematological and immune variables in patients and comparing them with the control group, it was found that there is a positive correlation between IL-1β and IL-18, as well as a positive relationship between white blood cells with the two types of immune variables.

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