21 May 2022
31 May 2022
The concentration of vitamin D in the follicular fluid was found to have a positive impact on the outcome of assisted reproductive technology (ART) by the intra cytoplasmic sperm injection (ICSI) among infertile women. This descriptive follow up study aimed to correlate follicular fluid vitamin D and serum estradiol hormone levels in to the success rate in (ART) by (ICSI). In this study, total of (100) infertile females. Blood and follicular fluid samples were taken from all participants for IVF-ICSI (on the day of HCG administration), and then the levels of serum Estradiol and follicular fluid 25-OH vitamin D were measured. The number and quality of oocytes, number of fertilized oocytes and clinical pregnancy were also evaluated. The serum Estradiol levels were measured by immunoenzymatic assay using the commercial ELISA kit, while the 25-OH vitamin D levels in the follicular fluid was evaluated by competitive chemiluminescnce method. Follicular fluid 25-OH vitamin D levels significantly affected (ICSI) outcomes with a mean of (16.6±1.78). The serum Estradiol levels also showed significant effects on (ICSI) levels with a mean of (2874.4±358.91), the grade (2) quality of oocytes showed statistical significance with a mean of (6.7±1.11) at (p=0.0001), while age, BMI, grades (1,3,4) oocyte qualities showed no significant effects. Higher levels of 25-OH Vit D in follicular fluid among patients undergoing ICSI/IVF along with high levels of serum estradiol yielded larger number of aspirated oocytes and fertilized oocytes and even better embryo quality and hence led to more success in ART pregnancy outcomes.