This study was carried out at Wasit Province / Iraq in cooperation with Fairouz Hospital/ unit of microbiology. Samples were collected from many places at Wasit province. It included 177 stool samples from Iraqi patients with diarrhea, and 30 healthy subjects as a control. The study was conducted during the period from October to December, 2021 to investigate the prevalence of intestinal parasites and bacteria. Only 54 stool sample were positive for E.histolytica and E.coli. The results showed that the percentage of parasitic infection among patients and control were 54 (30%) and 0 (0%) respectively, with significant differences. For the parasitic infection, the higher rate of infection was recorded for the group (1-10) years which reached 27.8%, while the lower was recorded for the group (60-70) years it reached 3.7% without significant differences. Regarding gender males rate was 72.3% and females rate was 27.8% in this study without significant differences. According to the residence, urban areas were reported 59.3% and rural areas were 40.7% without significant differences. Correlation between E.histolytica parasite infection and E.coli bacterial infection in patients group were that 100% and revealed significant differences.