Earthworms are widely used as ingredients for medicinal herbs to cure various diseases in the world of traditional medicine. Earthworm extract has antimicrobial, antipyretic, anti-inflammatory, fibrinolytic, and antioxidant abilities. Based on previous research, Eudrilus eugeniae extract has anti-inflammatory activity similar to aspirin. Earthworm paste can cause a reduction in inflammation, normalization of the number of erythrocytes and leukocytes, as well as differences in levels of neutrophils, lymphocytes, eosinophils, hemoglobin, and serum biochemical content, as well as acid electrolytes in cells. Coelomic Fluid Eudrilus eugeniae has similar effectiveness to Indomethacin, so it can be ascertained that coelomic fluid has potential as an anti-inflammatory. This study aims to determine the effect of Eudrilus eugeniae extract and hydrogel on the wound healing process. This study used the independent variables, namely Duoderm, Nacl, Ethanol Extract and Coelomic fluid. The dependent variable is inflammatory cells in the wound healing process. The sample of this research was Rattus norwegicus. Collecting data from the size of the wound which was measured using a caliper every day 0, 7, 14, 21, and 28, the results of this study showed that the positive control had 98.5% effectiveness of wound healing, treatment group 2 had 93.9% effectiveness, treatment group 1 had 85.3% effectiveness, and in the negative control only 69.1%, For inflammatory cells,on microscopic observation, it was found that on day 0 there were no inflammatory cells, on day 14 inflammatory cells such as PMN and Leukocytes were seen, and inflammatory cells had been seen reduced in day 28.