Infertility is divided into primary, secondary and idiopathic infertility. Idiopathic infertility is infertility that the underlying factors cannot be explained. This study aimed to find alternative support for the examination of undetermined male infertility tests (idiopathic) by analyzing the status of sperm chromatin and the quality of sperm. This study was an observational study with a total sample of 121 consisting of 93 male infertility patients and 28 samples as normal controls. Data collections of patients and sperm samples were conducted at RSIA Gunung Sawo and Hermina Pandanaran Hospital Semarang Indonesia. Sperm analysis was done by macroscopic and microscopic examination. Chromatin spermatozoa examinations were performed by Aniline Blue staining for chromatin maturation and Toluidine Blue staining for chromatin integrity. The ratio of immature chromatin in asthenozoospermia and oligoasthenozoospermia samples were higher than normozoospermia samples. Mann-Whitney test result showed a significant difference between the ratio of immature chromatin asthenozoospermia and oligoasthenozoospermia compared to normozoospermia from fertile men (p < 0,05). There was a significant difference in the level of poor sperm chromatin integrity between the infertile samples (asthenozoospermia and oligoasthnenozoospermia categories) compared to the fertile men with normozoospermia category (p <0.001). The level of integrity of sperm chromatin has a positive correlation with infertility in men (p < 0.001, r = 0.454). Sperm chromatin maturity and integrity in infertile patients with asthenozoospermic and oligoasthenozoospermic condition was lower significant than fertile men with normozoospermic caterogory.