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25 Sep 2021 (Vol 44 , Iss 05 )

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31 Oct 2021 (Vol 44 , Iss 05 )

Journal ID : TMJ-11-08-2021-10574
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Title : Calcium Ionophore as Oocyte Activator to Fertilization Rate and Cleavage Level on Kacang Goat Post Intracytoplasmic Sperm Injection (ICSI)

Abstract :

Intracytoplasmic sperm injection (ICSI) a method in assisted reproduction technology by injecting a single spermatozoa directly into the cytoplasm of a mature oocyte via micromanipulation. The capacity of ICSI as a therapy for almost any type of spermatozoa and oocytes has made it the most successful treatment for infertility. Despite its high success rate, fertilization failure in ICSI still occurs this due to the quality of oocyte and sperm so that the oocyte cannot be activated. This research aimed to know the effect of calcium ionophore supplementation in Kacang goat’s oocyte after ICSI in fertilization rate and embryo development. This research was divided into 2 groups, control group: oocyte was fertilized with ICSI method and treatment group: oocyte was fertilized with ICSI method and supplemented in calcium ionophore 5.2 µl/ml during 15 minutes. Fertilization rate and embryo development at embryo 2 cells and 4 cells were observed under inverted microscope. Based on the statistical analysis it demonstrated there were significant difference in fertilization rate and embryo development between control group and treatment group (p<0.05). Fertilization rate in treatment group (3.56±1.42) was higher than control group (1.56±1.128). The embryo development from zygote to embryo two cells was higher in treatment group (3.56±1.42) than control group (0.78±0.09). The embryo development from embryo two cells to embryo four cells was higher in treatment group (3.33±1.12) than control group (0.56±0.26). In conclusion the used of calcium ionophore 5.2 µl/ml can activate Kacang goat’s oocyte after ICSI and improve fertilization rate and maintain embryo development.

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