Type 2 diabetes constitutes a major threat to public health, and non-ideal control leads to chronic hyperglycemia. This kind occurs due to the resistance of the body's cells to the action of insulin hormone which is caused by multiple factors, most notably the overweight and lack of physical activity, or may be due to the lack of production of a sufficient quantity of insulin by the pancreas or both. Current study was aimed to observe the total branched chain amino acids, valine, leucine, isoleucine, insulin, C-peptide and insulin resistance and their relationship in type 2 diabetes mellitus patients. One hundred types 2 diabetes mellitus patients and an additional 75 non-diabetic controls were recruited. Age and body mass index of the subjects were recorded. Plasma total branched chain amino acids, valine, leucine and isoleucine, serum glucose, HbA1c, insulin and C-peptide were measured from the collected plasma and serum samples and HOMA-IR was calculated. Total branched chain amino acid, valine, leucine, isoleucine, the levels of serum glucose, HbA1c, insulin, c-peptide, and HOMA-IR showed significant increase in type 2 diabetes mellitus patients as compared to control group (P<0.05). In addition, an increase level of BCAAs was positively associated with high HbA1c level, serum insulin, C-peptide and insulin resistance in type 2 diabetes mellitus.