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Journal ID : TMJ-04-11-2022-11440
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Title : Biomechanical evaluation of the effect of acellular amniotic membrane loaded by autologous platelet rich plasma on bone healing in a dog model

Abstract :

It may be possible to hasten the healing of bone tissues using a platelet concentrate composed of cytokines and structural glycoproteins entrapped within polymerized fibrin meshwork. Due to the availability of numerous growth factors, the amnion membrane has also attracted significant attention in the field of bone regeneration. The aim of the present study was to evaluate the effect of an amniotic membrane loaded with platelet-rich plasma on the healing of the fracture. These effects were demonstrated objectively by biomechanical testing. Fifteen mongrel dogs were used in this study. The animals were divided into three groups (n = 5 per group). A transverse osteotomy was induced in the midshaft of the left tibia of all animals, and then two groups of them were treated with bioscaffold (amniotic membrane) and platelet-rich plasma, while the third group was left without treatment. All fractured tibias are followed by a bone plate with screws as the following: The first group was treated with acellular amniotic membrane only by wrapping it around the fracture site. The second group was treated with acellular amniotic membrane accompanied by platelet-rich plasma, while the third group (control) was left without treatment. On day 56, all of the dogs had biomechanical tests done with the Universal Testing Machine, which was placed between the ends of the bones at the osteotomy site. The average maximum loads in Group (AM+PRP) were higher than in other groups (2.14 ± 0.050). The values of the (AM) group were (1.52 ±0.086). While fracture values were found to be lower in the control group than in the (AM) and (AM+PRP) groups (1.05 ± 0.050). It was found that bones recovered with AM+PRP (group) had the highest value and tibias recovered without treatment (control group) had the lowest value. Of the current study was that the application of amniotic membrane loaded with platelet rich plasma to the fracture site significantly improves the biomechanical strength of the fracture union.

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