Even though its gold standard status, culture method is time consuming and expensive to use when diagnosing UTI. For this reason, rapid and accurate biomarkers are needed to diagnose UTIs and their causative more quickly. The prediction of UTI and finding specific pathogens requires the identification of a biomarker which is more precise as they have extended their list. One hundred patients were selected from the Ramadi Teaching Hospital by urologists in expert urologist clinics according to inclusion criteria for UTI cases. Fifty apparently healthy subjects with the same ages and sexes were included in the study as control group, Human Interleukin- 6 and Interleukin-8 evaluated in blood samples, Elastase Procalcitonin and CRP were evaluated in urine samples, by using ELISA technique. The females were 68% and males were 32%, the mean age of patients was 35.95±12.3, and for control was 33.0±10.33, Escherichia coli isolated from 21%, followed by 13% and 12% for Staphylococcus epidermidis and Staphylococcus aureus while 4% for Klebseilla pneumonia, all included markers were significantly higher in patients than controls. The AUC for CRP was 0.998, sensitivity and specificity of the test were 98% and 97%, respectively and the highest levels of IL- 6, CRP, PCT and Elastase were recorded with K. pneumonia. All markers used in this study have a significant differences between patients and control, CRP was imperative markers in urinary tract infection followed by IL-8 and procalcitonin.