Abstract :
The use of plant extracts in the biosynthesis of nanoparticles displayed superiority over other biological agents and had and promising strategies for anticancer treatment. This study explored the antitumor effect of biosynthesized copper nanoparticles shelled with turmeric, sumac and vitamin B12 as core-shell or core-double-shell nanostructures at genetic and epigenetic levels and evaluate these nanomaterials side effect on hemopoietic, liver and kidney status in comparison to CuO nanoparticles on tumor bearing mice. All nanomaterials synthesis and characterization were done to confirm the successful synthesis. To conduct this study female mice were injected with Ehrlich Ascites carcinoma cells and then treated with either one of the nanostructures or CuO nanoparticles. Antitumor effect of nanomaterials was evaluated by measurement of tumor volume and serum CA15-3 level. miRNA-34a, and P53 RNA expression were also measured. Side effect of these nanomaterials’ treatment were assessed by screening the complete blood picture and measurement of liver and kidney functions. All nanomaterials significantly diminished tumor growth and CA15-3 level. Although, it seems that CuO nanoparticles showed the most significant reduction in tumor volume, the other nanostructures have higher impact on miRNA-34a and p53 RNA expression moreover, they attenuated the hematological, liver and kidney biomarkers more than CuO NPs did. Using turmeric nanostructures has more powerful effect than Sumac nanostructures otherwise adding vitamin B12 as second shell improve the nanostructures efficiency. The biosynthesized nanostructures induced much deeper effect on epigenetic and genetic levels and attenuated the side effects of nanomaterial treatments than chemically synthesized CuO NPs.
