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02 Dec 2022 (Vol 45 , Iss 10 )

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30 Nov 2022 (Vol 45 , Iss 09 )

Journal ID : TMJ-13-03-2022-11193
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Title : Antibacterial activity nanoscale( Zinc , Silver , Titanium ) of Staphylococcus aureus isolated from dental decay

Abstract :

The current study aimed to know the bacterial causes of dental caries, and for this purpose, for both sexes, at different ages ranging from (8-50) years, Then they were collected and transported directly to the laboratory and the samples were planted by plotting on plates of nutritious acar and then acaral blood and mkonkiAnd in solid saline mannitol medium under aerobic conditions, the type Staphylococcus aureus appeared at 248 isolates, or 31%. The sensitivity of the bacteria isolates under study was tested against 3 types of ntibioticsClindomycin, Metronidazole, Amoxicillin. Where wasolates of bacteria showed-S. aureus Resistance against all antibiotics. For isolates of Streptococcus It showed a difference in the response to antibiotics. The sensitivity of bacterial isolates to some types of manufactured nanomaterials was tested. Zinc, Sliver, Titanium with different concentrations, where the concentrations (100, 75, 50, 25) were used. The presence of the inhibition circuit indicated that zinc nanoparticles are a good antibacterial in varying proportions. Not all isolates appeared in response to silver and titanium nanomaterials, after which the treatment was treated with the synergistic effect. For nanomaterials on bacterial isolates, they gave better results than using them alone. The results showed the variation in the response of the isolates to the synergistic effect of the antibiotic with the nanomaterials and the difference in their sensitivity and resistance from one isolate to another. The results of the study showed using the technique of RT-PCR differentiated response to virulence factors protein A, haemolysin, TSST to bacterial isolates S. Aureus For the effect of zinc nanoparticles used in concentrations (2,1,0.5,0.25mg). And we got the highest inhibition at the concentration (0.5 mg). The results of the current study confirmed the response to the virulence factor (haemolysin) for the effect of zinc nanoparticles used in concentrations (2,1,0.5,0.25mg), and we obtained the highest inhibition of gene expression at the concentration (0.5 mg). The results of the current study confirmed the response to the virulence factor (Protein A) has the effect of zinc nanoparticles used at concentrations of (2,1,0.5,0.25mg), and a decrease in the gene expression of the factor was noted and the best inhibition was observed at the concentration (0.5 mg). In the current study, we obtained the effect of gene expression of virulence factor (TSST-1) in response to zinc nanoparticles used at different concentrations (2,1,0.5,0.25mg), and a decrease in the gene expression of the agent was noted and the best inhibition was observed at the concentration (0.5 mg).

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