04 Dec 2023
30 Nov 2023
Dental biofilm considered as the primary etiology for periodontal diseases and its early colonizers are of great importance in the succession stages of biofilm formation such as Streptococcus oralis. Nowadays there is a need to find natural substances from plants with antibacterial activity as an alternative to Chlorohexidine. To determine in vitro antibacterial effects of alcoholic Commiphora Myrrha and Ziziphus spina-christi leaves extracts alone and in Combination against Streptococcus oralis. Supragingival dental plaque samples were taken from 15 subject with dental plaque induced gingivitis then morphological, microscopical examination, biochemical tests and Vitek 2 were used to confirm identification of Streptococcus oralis. The Commiphora Myrrha and Ziziphus spina-christi leaves extracted by using ethanol alcohol. The susceptibility of bacteria against the extracts, the minimum bactericidal concentration and the minimum inhibitory concentration were determined separately and in Combination compared with chlorhexidine gluconate 0.2% and deionized water. Commiphora Myrrha and Ziziphus spina-christi leaves ethanol extracts had considerable antibacterial effects against Streptococcus oralis with various degrees of growth inhibition zones. It was shown that Combination extracts was the most effective compared to Chlorohexidine then Myrrha and lastly Ziziphus leaves extracts. The minimum inhibitory concentrations of the extracts ranged from (0.2-0.6 g/ml). The minimum bactericidal concentration of alcoholic extracts ranged from (0.4- 0.8 g/ml). The antibacterial activity of Alcoholic combination extracts was the highest antibacterial activity with all concentration against Streptococcus oralis than Myrrha and Ziziphus leaves extracts were even higher than Chlorohexidine when used at higher concentration, so it can be used as an alternative to Chlorohexidine.