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Journal ID : TMJ-22-11-2023-11587
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Title : Aminoglycoside resistance phenotype and genotype characteristics in Escherichia coli and Klebsiella pneumoniae: Interaction with an external clinical patient with an intraabdominal infection in Cipto Mangunkusumo National General Hospital (RSCM)

Abstract :

Southeast Asia has the highest risk of antimicrobial resistance (AMR). An intra-abdominal infection (IAI) in Indonesia is caused by E. coli and K. pneumoniae. This cross sectional descriptive analytical study, which conducted at Cipto Manunkusumo National General Hospital (RSCM) Jakarta, Indonesia, aimed to analyze aminoglycoside resistance in these bacteria and its impact on clinical digestive disorders. Samples were collected from IAI diagnoses between January 2019 to December 2020, and tests were performed in 2022-2023. The samples were sub-cultured on MacConkey media (35-37oC, 18-24 hours). The resulting colonies were identified with Gram staining and VITEK2 system. Isolates were tested for amikacin/gentamicin resistance with E-test. Of 79 isolates, 72.2% were E. coli and 27.8% were K. pneumoniae. A small percentage (6.3%) exhibited armA gene resistance. Patients with gentamicin resistance had a higher mortality rate (24.1%) compared to those without (14.7%), while amikacin resistance correlated with even higher mortality (40%). Risk factors like qSOFA, age, and amikacin resistance were statistically linked to clinical outcomes. The factors mentioned should be used cautiously as independent predictors, as they interact with external results. The researchers suggest that subsequent research should focus on improving PCR processes for greater accuracy and reducing errors.

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