Abstract :
This study was conducted to ameliorate Cu nanoparticles toxicity by biosynthetic methods using turmeric, Sumac, and vitamin B12 in core-shell or double shell nanostructures. Cu nanoparticles were biosynthesized and then used to form core-shell nanostructures with turmeric, sumac, and vitamin B12 (TurCu, SumCu, Cu+B12). TurCu and SumCu were reused for the biosynthesis of double core-shell nanostructures with vitamin B12 (TurCu+B12. SumCu+B12). Characterization of nanostructures and CuO Nanoparticles were done using XRD, TEM, SEM, DLS, and zeta sizer. Female mice were used to determine LD50 of acute exposure to intraperitoneal injection of different nanostructures. To evaluate the chronic toxicity of nanostructures, other group of female mice were exposed to daily injections of 10, 20, or 30 mg/kg b.wt for each and compared to the same doses of CuO nanoparticles and normal control group. CBC, liver, and kidney functions were measured in serum and Cu deposition in liver and kidney tissues was determined. Characterization of nanostructures indicated the successful synthesis of core-shell and double core-shell form within the nanoscale. Biochemical analyses revealed that injection of CuO nanoparticles caused dose-depended toxic effects compared to control group. On the other hand, injection with any of the core-shell structures significantly (P<0.05) modulated CBC, liver function, and kidney function tests with a non-significant reduction in liver and kidney Cu deposition compared to CuO groups. TurCu has the least toxic effect compared to the other core-shell nanostructures. Meanwhile, vitamin B12 as a double core-shell has a positive effect on CBC but negatively affected liver and kidney toxicity.
