08 Oct 2023
31 Oct 2023
Good scientific practices are a set of individual and organizational actions and behaviors based on fundamental values of science that express the principles and responsibilities that scientific integrity entails. Good scientific practices sustain responsible conduct in research because they maintain the pattern of conduct provided by regulations and declarations considered binding, which are characterized by promoting ethical principles, who a rigorous practice contribute to scientific progress.
The aim of the present study was to evaluate Hepatitis E Virus (HEV) contamination in shellfish from Oualidia lagoon (Moroccan Atlantic coast). The survey was carried out on 104 shellfish samples (87 oysters and 17 clams) collected at four sites in Oualidia lagoon, monthly between November 2015 and February 2017. Enteric viruses were detected by real-time reverse transcription polymerase chain reaction (RT-PCR) according to the ISO/TS 15216 method. HEV was not detected in any shellfish samples. This qualitative study on contamination by enteric viruses (HEV) highlights the value of regular monitoring of the viral risks associated with bivalve mollusks intended for human consumption.
Breast cancer is the most common cancer in women worldwide. It has many causes and one of these is genetic, as many genes are implicated in the generation of this type of cancer. The vitamin D3 receptor is one of these genes and has been shown to be involved in the development of breast cancer through a polymorphism. It is in this context that we conducted our study in which we investigated the relationship between the Fok1 and Taq1 polymorphisms, breast cancer and plasma vitamin D concentrations in Moroccan patients. Our study included 98 women, 53 of whom were ill and 45 healthy. We tested for two types of VDR polymorphisms, Taq1 and Fok1, by real-time PCR. Vitamin D status was assessed by the electrochemiluminescence method. Our work concluded that there is no correlation between Fok1 and disease stage and a probable Fok correlation. It was shown that there is a significant correlation between vitamin D and Fok1. In conclusion, vitamin D deficiency appears to be related to the Taq1 polymorphism which may increase the risk of developing breast cancer in Moroccan women.
Sodium-glucose co-transporter 2 (SGLT2) inhibitions reduces cardiovascular events in type 2 diabetes (T2DM) and is associated with a reduction in left ventricular (LV) mass index. However, the impact on right ventricular (RV) remodeling is unknown. Accordingly, the objective of this study was to assess the impact of SGLT2 inhibition on RV parameters and function in patients with heart failure with preserved ejection fraction. Although there have been many advances in noninvasive RV imaging during the past decade, echocardiography remains the most accessible and easiest method to assess RV volumes and function during follow-up. This study aimed to examine the effect of empagaliflozin on RV function assessed by FAC, TAPSE, RVOT SE, TDI and RV speckle tracking in patients with HFpEF. This study was carried-out on 40 patients suffered from HFpEF and with definite diagnosis of HFpEF; the patients were collected from Al-Azhar –University Hospitals (Cairo, Egypt). All patients included in our study subjected to full data Echocardiography and both conventional parameters (RV/ LV diameter ratio, RV FAC, TAPSE, TV TDI s' velocity) and RV speckle tracking parameters (RV GLS) before treatment and after 6 months of medical treatment that include empagaliflozin. All echocardiographic parameter were assessed in blinded fashion. At baseline, the mean of the following percent of LL edema (90%), systolic blood pressure was (159 mmHg), LVH (87% of patients) and TAPSE (17.02), RVGS (-17.17), RV free wall strain (-21.17), FAC (33.85%), RV/LV ration <1 (47.5%), RV/LV >1 (52.5%), RVOT SE (4.55 mm), TDI e` lateral tricuspid annulus (7.22 cm/s), TDE s` TA systolic velocity (9 cm/s). While after using the empagaliflozin the mean was percent of LL edema (7.5%), systolic blood pressure was (141.37 mmHg), LVH (70% of patients) and TAPSE (20.97), RVGS (-20.7), RV free wall strain (-24.72), FAC (36.90%), RV/LV ration <1 (100%), RV/LV >1 (NOT RECORDED), RVOT SE (5.52 mm), TDI e` lateral tricuspid annulus (13.22 cm/s), TDE s` TA systolic velocity (13 cm/s) respectively. In both groups, there was significant correlation between effects of empagaliflozin using on RV improvement changes from baseline to 6 months. This study concluded that with treatment empagaliflozin for patients with HFpEF enhance the RV systolic function measured by speckle tracking RV global strain and RV free wall strain, RV FAC, RVOT SE and TDI.
Pulmonary fibrosis (PF) is a chronic and progressive lung disease especially after severe acute coronavirus disease 2019 (COVID-19) infection, characterized by the formation of dense fibrous connective tissues. The present study is conducted to investigate the anti-fibrotic effects of eugenol oil (Eug) and eugenol nanoemulsion (Eug-NE) against pulmonary fibrosis induced by bleomycin (BLM) in rats. Lung fibrosis is induced by BLM in adult male rats. The animals were given Eug oil, Eug-NE, or dexamethasone (DXA) orally for 4 weeks, daily, starting one day after BLM. Lung injury, edema, and collagen deposition were determined. Some biochemical measurements, gene expression of apoptotic markers, immunohistochemistry, and histopathological examination of lung tissue are performed. The results revealed significant deterioration in lung tissues by BLM that is confirmed markedly by the pro-inflammatory, pro-oxidative, profibrotic, and pro-apoptotic conditions in lung tissues. However, treatment with Eug-NE potentially exhibited anti-fibrotic effects in lungs by downregulation of MMP-9, TGF-β and hence fibronectin expression. It also have the potential to cease the vicious cycle of epithelial injury caused by their anti-inflammatory and antiapoptotic effects. The effects of eugenol nanoemulsion were better than those of DXA. In conclusion the present study provides new insight into the use of natural oils in nano-emulsified formulations to treat pulmonary fibrosis which is one of the currently spread disorders as a result of severe COVID-19 infection.