10 Mar 2024
31 Mar 2024
This In-Vitro study was conducted to compare the wasted dentin during separated instrument (SI) removal using two different methods (ultrasonic versus tube technique), and to determine the mechanical strength of the dental root after this procedure. Teeth used in this study (n=40) were all single-rooted. Each tooth was first fixed in a putty silicon base, then an instrument (Protaper 25#) was intentionally separated in middle and apical third of root canal. In group I (n1=20), ultrasonic tips were used to retrieve separated instruments (SIs), while Zumax kit were used in group II (n2=20). A CBCT image were taken for all teeth before and after the removal attempt in each group, and amount of losted dentin during the procedure was compared. All the teeth were prepared for mechanical testing in Testometric machine to measure the required force for root fracture. Statistical analysis was done using PASW Statistic® 18. Results showed that tube technique removed more dentine than ultrasonic tips when SI was in the apical third of root canal, while no statistical difference was found between the two methods when SI was in the middle third. Also fracture resistance declined significantly with tube technique comparing with ultrasonic tips.
Acute kidney injury (AKI) is a disruption in kidney function that is acute and generally reversible. Study showed that AKI developed in 26.9 patient and incidence of stage 2 and 3 reached 11.6% during 7 days of treatment in the Pediatric Intensive Care Unit (PICU). Renal Angina Index (RAI) recently proposed to be tool for stratification of critical patient based on risk for AKI. Early screening tool such as RAI was believed to be helpful in assessing mortality rate and optimal treatment especially for pediatrics patient in critical condition and there was limited resources about RAI to date. To determine the relationship between the Renal Angina Index and mortality in critically ill children. A retrospective study in critically ill children who were treated in tertiary public hospital. Renal Angina Index is calculated in the first 24 hour of admission. Samples were collected from June-July 2019. Of the 101 subjects, 58.4% of them were male, largely distributed in 1-12 months group (31.7%), 64.4% with RAI ≥ 8 and mortality rate of 38,6% There was significant association between Renal Angina Index and mortality (p<0.05) with adjusted odd ratio 3,191 (95% CI: 1,266-8,042). Renal Angina index is significantly associated with mortality in critically ill children.
Human papillomavirus type 16 (HPV 16) is involved in the infection and transformation of malignant cells leading to the development of cervical cancer in 70% of cases. The HPV 16 genome encodes six early proteins including the HPV 16 E6 oncoprotein, which deregulate cell proliferation by interfering tumor suppressor p53.As well, HPV 18 E7 oncoprotein dysregulate pRb. Consequently, the cell cycle is disrupted inducing DNA damage, genomic instabilities as well as structural or numerical chromosomal aberrations. This study aimed to explore the karyotype of rat myoblast murine L6-transfected cells with HPV 16 in order to identify any chromosomal aberrations. Karyotype analysis was performed by standard G banding technique of HPV 16 L6 transfected rat myoblast cells and control L6 rat myoblast cells. Electroporation was used for the HPV 16 DNA exogenous transfection of rat myoblast cell L6. The chromosomal map reveals chromosomal aberrations illustrated in the translocation ideogram. The results shown that in approximately 80% of the cells there is a copy of a chromosome resulting from a Robertsonian translocation between the long arm of two chromosomes and 50% of the cells show double minutes. Our findings indicate a highly significant link between HPV 16 and chromosomal aberrations of infected cells. That suggests a very likely link between these and cervical cancer. Further studies on human cell samples are needed to provide better evidence for this binding.
Diabetic foot syndrome (DFS) is a long-term consequence of diabetes caused by both micro and macrovascular issues. Due to the paucity of information and researches on DFS in Basrah, Iraq, in this study we examined and compared the role of insulin resistance (IR), oxidant/antioxidant status, some adipokines and glycoproteins in Type 2 diabetic patients with DFS. This was a case-control study involving 89 subjects, consisting of 45 DFS subjects and 44 healthy subjects. Their fasting Insulin, chemerin, Zonulin, ceruloplasmin, α2-macroglobulin, Malondialdehyde (MDA) and Total antioxidant capacity (TAC) were determined by ELISA methods. Demographics, glucose was assayed on UV-Vis Spectrophotometer, HbA1c measurement was determined using the Bio-Rad D-10® HPLC analyzer, and homeostasis model assessment for determined of insulin resistance (IR). When compared to controls, glucose, Insulin, HOMA-IR, HbA1c, Chemerin, Ceruplasmine, Zonulin, α-2Macroglobulin and Malondialdehyde levels were significantly elevated (p<0.01), while total antioxidant capacity was lowered (p<0.01). These measures could potentially be employed as prognostic biomarkers in both men and women with DFS, according to the area under the curve (AUC). The associations of insulin resistance (IR) with examined adipokines and glycoproteins were not significantly different, which may shed new light on the role of IR as an etiological cause of DFS and suggest that it could be a better monitoring index in women and men with DFS.
The problem of unethical behavior in science and academia has grown to become a topic of concern in areas related to the generation of knowledge and the training of future researchers, at the level of students to the lack of training at the institutional level and in professionals due to the growing demand to publish, among other factors. Describe the importance and practices that university students have training in recognizing elements of scientific and academic misconduct. A descriptive cross-sectional study was conducted on 33 students from Colombian and Mexican universities, both public and private, through the application of a survey constructed by 12 questions related to their practices of scientific totality in the recognition of probable errors of misconduct and their normalization whether intentional or not. For the analysis, the Wizard 2.0® program was used. Twenty-two (22) Students recognize the importance of scientific integrity for their research and professional training, in addition, that institutions should be permanently trained in these topics. The needs design a model for complaints and sanctions; but also, the universities reinforce the value of training quality in scientific and academic integrity.